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Graphics / CAD / SVEDBERG 7.0.2
SVEDBERG 7.0.2
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SVEDBERG 7.0.2 Description
SVEDBERG is a Windows program for whole-boundary analysis of sedimentation velocity data.
SVEDBERG is a Windows program for whole-boundary analysis of sedimentation velocity data. It fits directly to the raw scans (usually spanning the entire run) to derive the sedimentation coefficient and molar mass (or, if you prefer, sedimentation coefficient and diffusion coefficient) for up to 6 independent species (mixtures of up to 6 components).
The program is designed to be easy to use, even for novices, yet it also offers many options for the 'power user' when they are needed. It uses multi-page analysis 'documents' and a familiar user interface with toolbars and buttons, making it similar to other Windows programs to reduce the learning curve. It also incorporates a number of intelligent 'wizards' that automatically accomplish tasks such as locating the meniscus position, removal of systematic noise ("jitter") and fringe jumps from interference scans, and even making reasonable initial guesses for the molecular parameters.
This is not a package that tries to do everything---its purpose is to implement one particular approach (analysis of single species and mixtures), do that very well and very easily, and do it in a highly reproducible and well-documented manner. It is not intended to be able to handle samples where the data is strongly influenced by dynamic reversible-association reactions (interacting boundaries). However when reversible interactions are strong enough that a reversible oligomer or complex can be treated as a single species then SVEDBERG is quite useful for identifying the molar mass (stoichiometry) of that species, which in turn can be a very useful input to analysis of data using other software acquired under conditions where the dynamic interactions are more significant.
The success of this approach is demonstrated by its use in 140+ known publications. More significantly, many of these are from intermittent or casual users of AUC, proving you don't have to devote years to learning data analysis to successfully answer important research questions with SV.
The program is distributed as "trial-ware"; it can be downloaded for a 60-day free trial period.
Major features:
<em>- Modern multi-document, Explorer-style user interface</em>
toolbar with standard buttons for file open, file save, etc.
multiple analyses can be open simultaneously,
Explorer-like navigation tree to move to specific analysis pages (analysis steps) and between analysis documents
click-and-drag setting of meniscus marker, data limits, cell base position, etc. [see image below
Allows direct conversion of a c(s) distribution from SEDFIT into an equivalent mixture model, allowing determination of true confidence intervals for all the species fractions and sedimentation coefficients simultaneously
when desired you can modify the constant f/f0 ratio constraints (for example using oligomer constraints instead) or try removing the constraint altogether to see whether the mass of a minor component can be determined independently
SVEDBERG can load the data range and other parameters used in the c(s) fit
Allows use of any of the current methods for handling systematic noise in the raw scans:
either fitting the TIN (time-invariant noise), scan differencing, or a simple baseline offset
for interference or pseudo-absorbance scans, either arithmetic removal of "jitter" (radially-invariant noise, RIN) at a fixed region of the cell or fitting of the RIN
Improved Lamm equation solution gives s, D, and M values for each species with theoretical accuracy of better than 0.1%, and will work for small peptides
Evaluates fitted parameter confidence intervals using any of three orthogonal approaches: F statistics, the bootstrap method, or the Monte Carlo method
For multi-species fits you can choose whether to fit the total concentration and species fractions, or to fit the loading concentration for each species
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OS: Windows 7, Win95, Win98, WinME, WinXP, Windows 2000, Windows 2003, Windows Vista
Tags: Sedimentation Rate Calculator - Analyze Sedimentation Rate - Molecular Mass Calculator - Sedimentation - Sediment - Calculate
User Reviews of SVEDBERG
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DCDT+ 2.3.4 DCDT+Oao is a WindowsT« program for analysis of sedimentation velocity (SV) data that implements the dc/dt method originally developed by Walter Stafford, and also implements fitting of the derived g(s*) distributions as a mixture containing of up t.. Download